Whole genome sequencing in diagnostic microbiology

Routine Use of Microbial Whole Genome Sequencing in Diagnostic and Public Health Microbiology

1 Department of Medicine, University of Cambridge, Addenbrooke’s Hospital, Cambridge, United Kingdom, 2 Clinical Microbiology and Public Health Laboratory, Health Protection Agency Microbiology Services, Addenbrooke’s Hospital, Cambridge, United Kingdom, 3 The Medical School University of St. Andrews, North Haugh, St. Andrews Fife, United Kingdom, 4 The Wellcome Trust Sanger Institute, Wellcome...

Whole genome sequencing in clinical and public health microbiology

Genomics and whole genome sequencing (WGS) have the capacity to greatly enhance knowledge and understanding of infectious diseases and clinical microbiology.The growth and availability of bench-top WGS analysers has facilitated the feasibility of genomics in clinical and public health microbiology.Given current resource and infrastructure limitations, WGS is most applicable to use in public hea...

Rapid bacterial whole-genome sequencing to enhance diagnostic and public health microbiology.

IMPORTANCE The latest generation of benchtop DNA sequencing platforms can provide an accurate whole-genome sequence (WGS) for a broad range of bacteria in less than a day. These could be used to more effectively contain the spread of multidrug-resistant pathogens. OBJECTIVE To compare WGS with standard clinical microbiology practice for the investigation of nosocomial outbreaks caused by mult...

Diagnostic value of exome and whole genome sequencing in craniosynostosis

BACKGROUND Craniosynostosis, the premature fusion of one or more cranial sutures, occurs in ∼1 in 2250 births, either in isolation or as part of a syndrome. Mutations in at least 57 genes have been associated with craniosynostosis, but only a minority of these are included in routine laboratory genetic testing. METHODS We used exome or whole genome sequencing to seek a genetic cause in a coho...

Whole Genome Sequencing: An Efficient Approach in Food Safety Management System